vector labs特约代理商|L-1000 CONCAN

Dissociated Retina Immunohistochemistry

Medium 45% HAMS F-12 (Gibco #11765-054) 45% DME (Gibco #10313-021) 10% FCS (HyClone) Insulin (5 mg/ml 1000X stock in H2 O with HCl) (10 ml/liter) L-glutamine (200 mM, Cellgro # 25-005-CI) (10 ml/liter) Penn/Strep (Cellgro #30-002

生物芯片实验手册（NIH）

50X TAE Buffer 40 ml Ethidium Bromide (10 mg/ml) 0.1 ml Water 960 ml 1000 ml Loading Buffer Glycerol (enzyme grade) 4.0 ml DEPC Water 0.9 ml Dye Solution* 0.1 ml 5.0 ml (*This solution is 0.25% (w/v) Xylene Cyanol

【转贴】immunofluorescence staining

, or clean them before use with ethanol.Mounting Media:For most microscopy: Vector Labs - Vectashield Mounting Media, Cat.# H-1000, 10 mls.For deconvolution microscopy: Molecular Probes - SlowFade Anti-fade kit, Cat. #S2828. (Vectashield contains some non