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上海玉博生物科技有限公司
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文献和实验Sample Preparation for Microinjection
during microinjection, solutions containing the markers should be filtered with a syringe filter (pore size 0.2 µm) whenever possible. Also, before loading the capillary, the sample should be centrifuged for 15 minutes at 4°C (10,000 x g). Peptides
Sample preparation (analytical gels)
containing urea (8 M), CHAPS (4 % w/v), DTE (65 mM), Tris (40 mM) and a trace of bromophenol blue. Hundred μl of the final diluted kidney cell sample was loaded onto the IPG gel strip. LIVER Protocole 1: Five frozen slices (20 μm x 5 mm x 10 mm
Sample preparation (preparative gels)
Human samples CEC (Colon epithelial cell): Within a maximum of 30 min, a large right or left colon tissue sample (5 x 7 cm) was prepared on ice in the operating room, rinsed with cooled phosphate buffer saline, pH 7.2 to further remove
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