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文献和实验and you are not able to scale up your isolation of protein to reach coomasie stainable levels,you must use a mass spec compatible silver stain protocol.You must only use methanol and acetic acid during the fixing step.You must not use any solutions containing
We currently use the SilverQuest Silver stain kit from Invitrogen. This method is faster than the method listed below. The rapid method works well for most applications. Click here for instructions to the SilverQuest kit Non-Kit based
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
in immunostained chromosomes were initially described by Silver and Elgin (1976). For further details on dissecting larval salivary glands, see Dissection of Larval Salivary Glands and Polytene Chromosome Preparation (Kennison 2008). MATERIALS
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