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文献和实验Protocols for the preparation of tumour cells for s.c. injections in mice
. The trypsinized cells are transferred into a 15 ml falcon tube and 10 ml medium added. After centrifugation (5 min., 1100 rpm), cells are resuspended in sterile PBS (12 ml) and centrifuged again. The cell pellet is then resuspended in 500-600 µl of PBS and kept
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
HeTube Mating with MSCV recipient
50% Glycerol (sterile) 10 ml 20% L-arabinose (filter sterile) 1 ml Carbenicillin at 100mg/ml (for pMSCV selection) AND antibiotic to select for your insert POUR YEGlu: 1 liter MilliQ water 5 g yeast extract 10 g NaCl autoclave and cool add
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