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文献和实验Utilizing Peptide SPOT Arrays to Identify Protein Interactions
Fmoc‐amino acids (set of 20 amino acid cartridges; Intavis AG) Amino acid stock solution: 2.3 g 1‐hydroxybenzotriazole hydrate (HoBt) dissolved in 20 ml 1‐methyl
Synthesis of Peptidomimetic Conjugates of Cyclic Nucleoside Phosphonates
ester hydrochloride (Aldrich) Ethan‐1,2‐diol (ethylene glycol) 1‐Hydroxybenzotriazole hydrate (HOBt; Aldrich
rpm. Pre-Hybridization Re-hydrate fixed embryos into PBT (PBS 1X; 0.1% Tween 20) by passing through the following solutions (1ml each, 3-5 min each step): 90% MeOH; 10% PBT 70% MeOH; 30%; PBT 50% MeOH
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