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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Isolation and Purification of Rabbit Osteoclasts
rabbit osteoclasts on resorbable substrates in vitro are an ideal model of osteoclast behaviour in vivo, and for some studies may be preferable to osteoclast-like cells generated in vitro from bone marrow cultures or from human peripheral blood
Assaying Phospholipase A2 Activity
PLA2 α (cPLAα) and for secreted PLA2 s (sPLA2 ) that are suitable for purified enzymes and for measuring activity in crude cell lysates and culture medium. The assay for cPLA2 α involves measuring the calcium-dependent release of radiolabeled sn
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