Kapa biosystems 代理商|KK2501 Kap

TaqMan Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Quantification and Identification of bcr-abl Transcr

Real-time TaqMan� polymerase chain reaction (PCR) assays allow quantification of the initial amount of target in a specimen, specifically, and reproducibly. The major limitation of TaqMan PCR assays is that they do not detect the size

【原创】血液中直接PCR,无需DNA提纯

文库里全部的变异株，那么就要建造一个更大的，并且很昂贵的实验室。这在目前的情况下，完全不现实。这个瓶颈问题可以很容易地通过KAPA Biosystems公司的乳状液膜技术（Emulsion Technology）来实现。其技术核心是在一个1ml的试管中，将含有表面活性剂和膜溶剂的油相和水相(内水相) 置于容器中,在高速搅拌下制成油包水型乳状液,再将此乳状液分散到另一种水溶液(第3 相)中, 就得到了水包油再油包水型乳状液膜。当乳状液分散到第3 相时,形成数亿万记的乳珠。这样，每个乳珠就变成了一个小的PCR反应

【分享】分子生物学资料全集

: high-fidelity, long-range and RT-PCRs 48 3.16 Nucleic acid templates 51 3.17 Mineral oil 54 3.18 Plasticware and disposables 54 3.19 Automation of PCR and thermal cyclers 55 Protocol 3.1: Phosphorylation of the 5
-end of an oligonucleotide 63