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文献和实验Design and Generation of Gene‐Targeting Vectors
(C ). In a conditional knockout allele (D ), the critical exon 2 is flanked by two Cre recombinase recognition sites ( lox P sites). Upon Cre expression, the critical exon is excised, leading to a reading frame shift upon splicing to the downstream
Using the Saccharomyces Genome Database (SGD) for Analysis of Genomic Information
highlights how to retrieve data using YeastMine, how to visualize genomic information with GBrowse, how to explore gene expression patterns with SPELL, and how to use Gene Ontology tools to characterize large?scale datasets. Curr. Protoc. Bioinform. 35:1.20
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM
with yeast. In DNA Cloning, Expression Systems: A Practical Approach, B. D. Hames and D. M. Glover, eds. (Oxford: Oxford University Press), pp. 169-203. Finley, R. L., Jr., and Brent, R. (1996). Two-hybrid analysis of genetic regulatory networks
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