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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
> > TEMED (ul) 30 > > > > seal gel w/ 2mls mix (-TEMED) + 2ul TEMED range of separation % gel bp 3.5
for 30'. • Thoroughly dry the pellet and resuspend in 6 ml TE. Transfer 5.5 ml to a 13 ml snap top tube and add 6.1 g CsCl plus 0.3 ml EthBr (10 mg/ml). • Spin in the Brinkman Speedfuge for10' at 6K and transfer the supernatant to a Quick Seal
This is a standard large scale prep.for plasmid DNA which gives a yield of 0.5-1.0mg.I have made some minor changes to the MHB protocol. Solutions Solution I,II,III from protocol D.1. Tris/EDTA pH 7.5 (optional) 20 ml 1 M Tris 7.5 4 ml
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