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上海玉博生物科技有限公司
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文献和实验Isolation of murine splenocytes
; from Mediatech, catalog# 10-013-CM) 100mL fetal bovine/calf serum 10mL 100X PSG (penicillin G sodium, streptomycin sulfate, L-glutamine; from Gibco, catalog# 10378-016) sterilize using 0.2μm filter; store at 4°
SOLID TUMOR CULTURES FOR CHROMOSOME ANALYSIS
Penicillin/Streptomycin solution, 1 ml L-Glutamine solution, store at 4 C. Fixative: 30 ml Methanol and 10 ml Glacial Acetic Acid, prepared fresh. Hypotonic solution 0.075 M KCl: 2.8 g Potassium chloride dissolved in 500 ml dH2O. VI. Reagents
MEDIA FOR EMBRYO CULTURE AND MANIPULATION
Ingredient % of Final Volume 1 x DMEM with 20mM HEPES buffer 80.8% Penicillin/Streptomycin (5,000 i.u/ml/5,000ug/ml) 1% L-glutamine (200mM, 100x) 1% non-essential amino acid (100x) 1% nucleosides (100x) 1% b-mercaptoethanol (0.1M) 0.2
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