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Method for Single-Cell Electroporation

vectors containing promoters appropriate for tissue type. We purify our plasmid DNA using Promega Wizard Plus MidiPreps DNA purification system (Promega, Madison, WI). We dilute purified DNA to 0.2-1 µg/µl and fill the micropipette tip with 0.6-1 µl

Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY

    1     18.5 min           24:0ME     3     22.3 min 3. Run your samples: PL, MAG, DAG, FFA, TAG. You may have to vary the amount depending on the amount of lipid you have. Start with dissolving the PL fraction in 50 µl of hexane

Chemical transformation

, too). Transformation of electrocompetent cells 1. Chill cuvettes on ice for 5 minutes. Thaw cells on ice. 2. Add 5 pg - 5 µg plasmid DNA in 1 µl to the cells. Mix by tapping the tube or swirling the contents with a pipet tip. 3. Transfer the DNA and the cells