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文献和实验Preparation of total RNA from whole blood collected in PAXgene tubes
processing tube, and discard the old processing tube containing flow-through. 11.Apply 500 µl Buffer BR4 to the PAXgene column, and centrifuge for 1 min at ³ 8000 x g ( ³ 10,000 rpm). Place the PAXgene column in a new 2 ml processing tube, and discard
Quantitation of Protein:Micro-Kjeldahl Method
【 Purpose 】 Study the principle and the operational techniques of micro-Kjeldahl method. 【 Principle 】 The Kjeldahl method measures the nitrogen content of a compound and may be used to determine
Isolation of micro-RNA (miRNA)
Adapted from the Instruction manual of Stratagene''s Micro RNA Isolation Kit (Catalog #200344) Overview There are two main methods for isolating RNA: phenol-based extraction and silica matrix or glass fiber filter (GFF
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