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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Isolation of Microtubules (Bovine Brain)
content of your sample using the Bradford protein assay ( Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard curve. Decant the remainder of the crude supernatant into a chilled beaker, and add an equal
Screening Major Binding Sites on Human Serum Albumin by Affinity Capillary Electrophoresis
A screening method is described for determining whether a drug or small solute has significant interactions at the two major binding sites on human serum albumin (HSA). This method uses affinity capillary electrophoresis (ACE) to perform
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