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文献和实验Mouse B cell isolation with magnetic beads
and incubate on ice for 15min; l Negative : 1.Resuspend cells with MACS to 400 ul and 80 ul B cell Biotin each spleen,incubate on ice for 15 min; 2. Add 300 MACS and 200 anti-biotin Beads each spleen, incubate on ice for 15 min.
by immunopurification of the CD19- or CD20-expressing cells with magnetic beads coated with an anti-CD19 or anti-CD20 antibody. The purified cells can be used for a wide range of biochemical analyses, including protein extraction for Western blot and immunoprecipitation
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
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