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文献和实验Ahr knockout genotyping by PCR
was generated in ES cells from a 129 mouse strain which has the “d” allele. The BL6 mouse which we have bred onto has the “b” allele. Progeny will be +/+=b/b, +/-=b/d, or -/-=d/d. Ahr KO using Amplitaq Gold Master Mix: 10 ul Perkin-Elmer 10
Ahr transgenic genotyping by PCR
, sterile water 4 ul mouse tail DNA Typically you will be genotyping several animals at once, so make a master mix of everything but the DNA. Aliquot 46 ul to tube and add 4 ul of DNA. Set the reactions up on ice, and immediately
ARNT transgenic genotyping by PCR
tail DNA Typically you will be genotyping several animals at once, so make a master mix of everything but the DNA. Aliquot 46 ul to tube and add 4 ul of DNA. Set the reactions up on ice, and immediately put them into the thermalcyler
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