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文献和实验Chromosome painting refers to the complete decoration of specific metaphase chromosomes with complex probe mixtures. The painting signal is obtained by fluorescence in situ hybridization (FISH) of such mixtures established from a number
The Use of M-FISH and M-BAND to Define Chromosome Abnormalities
Multicolour fluorescence in situ hybridisation (M-FISH) and multicolour banding (M-BAND) are advanced chromosome painting techniques combining multiple chromosome- or region-specific paints in one step. M-FISH identifies all chromosomes
(4 ,5 ). Pinkel et al. (1988) used in situ hybridization of 120 fluorescent DNA probes (FISH) along the entire length of chromosome 4 to generate a specific painting probe for this chromosome (6 ). By the early 1990s, whole chromosome painting probes (wcp
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