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文献和实验FREEZE-CRACK b-GALACTOSIDASE STAINING PROTOCOL
FREEZE-CRACK b -GALACTOSIDASE STAINING PROTOCOL FIRE LAB 1. Prepare humidity chamber: line large square plate with humid paper towels and place 4 5ml plastic pipets across chamber to support slides. Place
Freeze-Crack P granule Staining Protocol
Freeze-Crack P granule Staining Protocol 1. Prepare humidity chamber: line large square plate with humid paper towels and place 4 5ml plastic pipets across chamber to support slides. Place aluminum block in dry ice
-crack to help liberate the DNA. Check that the solution actually freezes. Overlay with a drop of mineral oil and incubate at 60°C for 60 minutes, followed by 95°C for 15 minutes. Cool to 4°C. Pipette 22.5 l of PCR master mix
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