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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO4 in 1 liter H20 Fix in 0.5% glutaraldehyde in PBS + Mg2+ or 2.0%- 4.0% paraformaldehyde in PBS + Mg2+ on ice for 30
until they can be delivered to the reference laboratory. Zeus Medium, a commercial product, is probably similar. 1.0 M potassium citrate buffer pH 7.0: dissolve 21.0 g citric acid monohydrate (or 19.2 g citric acid anhydrous) in 30 mL of hot deionized or distilled
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