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文献和实验Flow Cytometric Analysis of p53-Induced Apoptosis
expressed p53 by transfection as well as for endogenous p53. The p53 protein is detected by intra-cellular fluorescent staining of p53 or by tagging p53 with GFP. The extent of apoptosis in cells expressing p53 is determined by cell cycle distribution using
Mutation of p53 Tumor Suppressor Gene in Hepatocellular Carcinoma
for a protein of 393 amino acids, which is expressed at relatively low levels in all tissues. p53 product is a 53-kDa phosphoprotein involved in the regulation of cell cycle, in DNA synthesis and repair, and in cell differentiation and apoptosis (see refs. 3 –
Identification of p53 Target Genes by Fluorescent Differential Display
Differential display (DD) is a method used worldwide for identifying differentially expressed genes in eukaryotic cells. The mRNA DD technology works by systematic amplification of the 3′ terminal regions of mRNAs. Using anchored primers
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