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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
. Mortar and pestle should be washed after every sample. 4. Working quickly before powder can thaw, pour or spoon ground mycelia into 15 ml round bottom Sarstedt tube (Sarstedt tubes #60.540, Princeton, NJ) containing 8 ml of E buffer at room
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
or CELLstart™ CTS™ substrate Attachment Factor Stem Cell Tested 0.22 μm pore size sterile filters BD Falcon T175 TC-Flasks 37°C water bath Appropriate tissue culture plates and supplies 实验步骤 1. Preparing
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
5) Allow the minced tissue to settle to the bottom of the centrifuge tube. Remove the supernatant until the total volume is ˜10 mL. Add 7 ml of collagenase solution to the centrifuge tube. 6) Put the conical tube
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