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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
. Mortar and pestle should be washed after every sample. 4. Working quickly before powder can thaw, pour or spoon ground mycelia into 15 ml round bottom Sarstedt tube (Sarstedt tubes #60.540, Princeton, NJ) containing 8 ml of E buffer at room
for 2 minutes. Use a vacuum aspirator to remove any drops of remaining medium adhering to the walls of the centrifuge tube or trapped in its neck. Freezing of Competent Cells Resuspend the cells gently in 20 ml of ice-cold Inoue
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
; BD Falcon 352340) (for Protocols 2-4) Centrifuge Flow cytometer (for Protocols 2-4) Forceps, 4-in. straight tips (Electron Microscopy Sciences 72991-4S) (for Protocol 4) Forceps, curved fine points Dumont #7 (Electron Microscopy Sciences
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