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文献和实验Methylated CpG Island Amplification
. Blot (in duplicate) onto nylon membranes. The easiest way to do this is to transfer the DNA mix to a 96 well plate and use a 96 pin replicator system (Nunc). Dry the membrane at RT for 30 min. Place the filters in 0.5M NaOH/ 1.5M NaCl solution
Methylated CpG Island Amplification (MCA)
. You should see a relatively strong smear, ranging from 300 bp to 2 kb. 3.2. Detection of Aberrant Methylation by Dot blot Analysis 3.2.1 Preparation of filters Transfer the PCR products to a new tube and add 2/3 volume of 5M NH4OAc
. You should see a relatively strong smear, ranging from 300 bp to 2 kb. 3.2. Detection of Aberrant Methylation by Dot blot Analysis 3.2.1 Preparation of filters Transfer the PCR products to a new tube and add 2/3 volume of 5M NH4OAc
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