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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
【转帖】Top 100 proteomics publications
Top 100 proteomics publications ----From Ion Channel Media Group：ttp://www.ionchannelmedia.com/websites.php 1. Gavin et al. (2006) Proteome survey reveals modularity of the yeast cell machinery. Nature 2. Bonetta et al. (2006) Protein
mm dish. This is about 100 ug protein for PC12 cells. After electrophoresis, electrotransfer to nitrocellulose membrane. I include in the SDS-PAGE prestained molecular weight markers to check the efficiency of the transfer
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