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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
) anti-hCD14-PE anti-hCD19-PE anti-hCD34-PE anti-hCD45-CyChrome Use corresponding PE-conjugated mouse IgG controls to evaluate specific labeling (see Step 19). CalPhos Mammalian
Isolation of Human and Mouse Neutrophils Ex Vivo and In Vitro
; Wartha and Henriques-Normark, Sci Signal 1:pe25, 2008). Neutrophils are, therefore, always a major focus of investigation for scientists all over the world. Isolation of neutrophils from either human beings or animals such as mice is a very common
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