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文献和实验S.11 a . (B ) Chromatogram of the conversion of S.11 a to uridine by treatment with 0.5 M TBAF in THF for 2 hr at 55°C. (C ) Chromatogram of mixed uridine and pyrene‐1‐carbonitrile commercial samples. Conditions: RP‐HPLC analysis is performed using
1200 series LC pump (Agilent Technologies) Synergi 4u Polar‐RP column, 80 Å, 4 µm, 4.6 × 50 mm (Phenomenex) API 4000
Synthesis of Oligodeoxynucleotides with 5′‐Caps Binding RNA Targets
of oxolinic acid. DNA probes with this cap, also known as ogOA cap, give melting point increases for duplexes with RNA targets and improve mismatch discrimination at the terminus. The cap is either introduced in one step, using a newly developed
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