invitrogen特约代理商|C6780 Coelente

荧光素酶和腔肠素Coelenterazine的选择及使用

光素，是海肾荧光素酶（Rluc）的作用底物，具有许多用途。可用于检测活细胞中钙离子浓度，基因报告分析，BRET（生物发光共振 能量转移）研究，ELISA，HTS，组织或细胞中超氧阴离子和过亚硝酸盐的化学发光检测，在460-470nm之间发射荧光。分子式：C26H21N3O3 分子量: 423.48 g/mol ，纯度：95% ，保存：-15°C避光，保存在惰性气体下。 腔肠素400a/ Coelenterazine 400a: 是BRET研究中海肾荧光素酶（Rluc）的首选底物，此腔肠素衍生

Measurement of Vascular Reactive Oxygen Species Production by Chemiluminescence

ROS detection. Lucigenin and luminol-enhanced chemiluminescent methods are described in the detection of vascular superoxide and peroxynitrite production and NAD(P)H oxidase activity. We also describe the use of new chemiluminescent probes, including

Monitoring Protein‐Protein Interactions in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)

PBS, pH 7.4 ( appendix 2A ) containing 0.5 mM MgCl 2 and 0.1% (w/v) glucose 50 µM coelenterazine‐h (Nanolight) in PBS, pH 7.4 ( appendix 2A ); prepare fresh from 1 mM stock