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文献和实验Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
( protocol 1 , step 22 and protocol 2 ) 3% (w/v) bovine serum albumin (BSA) (Merck Millipore, cat. no. 112018) in 0.01 M phosphate‐buffered saline (PBS
are indicated. Nucleotides that differ from the theoretical VE-1 binding site (VE-1 subs) are indicated in red. (G) DNA sequences selected in vitro from a randomized DNA library (N10 ) for its interaction with VE-1 by CAST assay. The number of sequences
of VEGF protein secreted from HEK 293 cells that had been transiently transfected with the corresponding ZFPs; measured with an ELISA assay kit (Chemicon, Temecula, CA). 'Vector' indicates a control vector that encode no ZFPs. An 'irrelevant ZFP
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