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文献和实验Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
5. Secondary antibody: anti-mouse IgG conjugated to horseradishperoxidase. 6. Enhanced chemiluminescent (ECL) reagents from Amersham(GE Healthcare, Piscataway, NJ). 7. Hyperfilm ECL: High performance chemiluminescence film(Amersham).
: Molecular Cloning Techniques (Brian White, MIT) Basic theory, definition and procedures with illustration ・ Western Blotting (Howell Lab) Procedures for preparation of cell lysate, gel, electrophoresis, transfer, antibody detection
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