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文献和实验Purification of acidic phosphatase from mustard seedlings
metal ions as cofactors and are common in animal tissues and bacteria. Acidic phosphatases are widely distributed in many organisms, including plants. They work optimally at ~ pH 5 without additional cofactors. The enzymes are classified as E.C. 3.1.3.2
Purification of acidic phosphatase from mustard seedlings
phosphatases are widely distributed in many organisms, including plants. They work optimally at ~ pH 5 without additional cofactors. The enzymes are classified as E.C. 3.1.3.2. In this experiment, we will extract an acidic phosphatase from seedlings
200ml PEG6000 40% 37.21 4.0g 20g 40g 60g 80g H2 O mQ 62.79 6.75g 33.75g 67.5g 101.25g 135.0g p=1.075. PMSF (store at -20o C) Conc. Stock 20ml C7 H7 FO2 S 100mM 174.2g/M
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