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Single and Double FISH protocols for Drosophila

the embryos to a 20 ml glass scintillation vial (see Note #5) containing a two phase mixture of 8 ml heptane, 2.5 ml 1XPBS and 250ul 40% formaldehyde. Shake for 20 minutes. 5. Using a 1ml pipetteman, draw up embryos (which are at the interphase), taking

Chromatin Immunoprecipitation Protocol for Histone Modification

  Introduction Chromatin Immunoprecipitation (ChIP) experiments are routinely performed in many laboratories around the world to examine the occupancy of proteins or chromatin modifications over particular stretches of the genome

Basic procedures for bacteria culture

  A. Phenol extraction of DNA samples Phenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated phenol is added to an aqueous DNA sample in a microcentrifuge tube. The mixture