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文献和实验not to disturb the microspheres. 27.Remove the tube from the magnetic separator and resuspend the microspheres in 1 mL of PBS-TBN by vortex and sonication for approximately 20 seconds. See Technical Note 4. 28.Repeat steps 25. and 26
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
., 1999). Back to top Procedure Experimental Design For each modified peptide to be tested, an unmodified peptide spanning the same residues should be included as a control. A control in which PBS alone is used instead of peptide
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
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