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文献和实验Procedures for In Vitro Production of Bovine Embryos
(E2758) BME amino acids solution (B6766) Bovine serum albumin, Fraction V (A3311) Bovine serum albumin, essentially fatty acid free (A6003) Calcium chloride (C7902) Epinephrine (E4250) DL-Lactic acid, sodium salt, 98% syrup (L-
concentrations of bovine serum albumin (BSA) ranging from 0.1mg/ml to 4mg/ml were made up in Eppendorfs. Using a 96-well plate, 25ml of each standard and of each unknown sample were pipetted into separate wells. Then 75ml of a solution of Lowry reagent
The Cell Environment (including types of culture medium)
since suppliers of large quantities of serum perform a battery of quality control tests and supply a certificate of analysis with the serum. In particular serum is screened for the presence of bovine viral diarrhoea virus (BVDV) and mycoplasma. Heat inactivation
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