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文献和实验How to make DEPC-treated water and Tris Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37℃.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris
Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing
(5 ). No splicing was detected in this study. However, the same extract preparation procedure, in conjunction with pre-mRNAs transcribed from cloned genes by SP6 RNA polymerase, was used to define optimal conditions for in vitro splicing (6 ). This system results
提取棉花RNA--RNA EXTRACTION METHOD FOR COTTON
and incubate with mild agitation on a rotary shaker at 100 rpm for 1.5 hr at 42℃.5.Add KCl to 160 mM and chill on ice for 1 hour.6.Centrifuge for 20 min at 12,000g.7.Filter supernatant through Miracloth and precipitate the RNA overnight in 2M LiCl at 4℃
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