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上海玉博生物科技有限公司
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
off the PBS. Add 100 µL of 1X PBS to each tube. Add 4 µg of antibody to one tube and 4 µg of preimmune serum to the other. Rotate for 4-6 h at 4°C. 31. Using the magnetic stand, aspirate the supernatants and wash the beads in each tube
protocol. a) Preclear cell lysates with protein G-sepharose. b) Incubate 200μg of pre-cleared lysates overnight with protein G coupled to the corresponding antibody (rotation overnight, 4°C, total IP volume 1.5 ml) c) Wash complexes 5 times
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
II. REFERENCES •Transfectol-LS Mammalian Transient transfection Kit. GeneChoice; TR10v2 KT-011106 III. EQUIPMENT, MATERIALS, AND REAGENTS • CO2 incubator, Forma Scientific incubator • BioSafety Cabinet, • Orbital Shaker, Forma
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