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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
, immunostaining of the chromosomes with a protein of interest, and counterstaining with Giemsa and Hoechst. RELATED INFORMATION Technical modifications incorporated into this protocol that have enabled more reproducible patterns of banding
Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage
in cells. The first step involves the synthesis of peptides (12–15 residues), where the phosphorylation site is centrally located, and a cysteine residue is incorporated at either the N- or C-terminus of the peptide to facilitate coupling
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
how the 6C technique can be incorporated with other techniques to discover all the chromatin regions in the nucleus that interact with a given gene or chromatin region of interest in a specific protein-dependent manner. Such information allows complete
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