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antibodies incorporated特约代理商| Anti-Centromere Protein Antibody 15-234-1ML
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Scientific Background Anti-Centromere Antibodies (derived from human CREST patient serum) are useful as controls in ANA diagnostics and also react with other species including mouse, rat, and hamster.
Format Antisera
Concentration Lot dependent
Clonality Polyclonal
Applications ICC, IHC
Host Species Human
Molecular Weight n/a
Species Reactivity Hamster, Human, Mouse, Rat
Antibody Registry ID AB_2939058
Physical State Liquid
Dilution Ranges IHC: 1:100
ICC: 1:100
Conjugate Unconjugated
Quality Control Serum obtained from an autoimmune patient was tested at a series of dilutions by immunocytochemistry on ethanol-fixed Hep2 cells that were in log-phase growth. The staining pattern obtained was consistent with the pattern expected for anti-centromere staining.
Usage Statement These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Country of Origin United States
Expiration 24 months from opening
Synonyms CREST, Centromere
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文献和实验Protein G Purification of Antibodies
1. Reagent and Materials(1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01)(2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrous3.273 g Na2HPO4.7H2Oq.s. to 1 liter with di-H2O(3) 0.1M Glycine, pH 2.83.75 g Glycine1.4 ml HCl
Coupling Antibodies to Protein A or G
1. use 2 mg of antibody per ml wet beads (use appropriate antibody/protein A or G combination). 2. mix antibodies with beads and bind at room temperature for at least 1 hr (on roller). 3. wash the beads twice with 10 volumes borate buffer, spin
Purification of Monoclonal Antibodies Using Protein A/G
A major breakthrough in immunology came with the discovery that large amounts of relatively pure monoclonal antibodies (mAbs) could be prepared from the fusion of B cells (secreting the relevant mAb) with a nonsecreting myeloma cell line
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