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文献和实验Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Mammalian Genomic DNA Miniprep Kit (Catalog Number G1N10) or equivalent. We recommend preparing DNA from the parent cell line that does not contain Target ID Library to use as a negative control for comparison with DNA from target-selected cells in PCR
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
information Moreover, as this procedure is quite complex, the use of commercial kits such as ApoTagTM (Oncor, Gaithersburg, MD, USA) and "In situ cell death detection Kit" (Boeringer-Mannheim, Germany), is recommended. Utilizing
protocol - Protocol for Annexin V-FITC Apoptosis Detection Kit (ab14085): 1. Incubation of cells with Annexin V-FITC 1) Induce apoptosis by desired method. 2) Collect 1-5 x 105 cells by centrifugation. 3) Resuspend cells
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