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上海玉博生物科技有限公司
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文献和实验Use either the whole blood or platelet-rich plasma (PRP) To obtain PRP, collect 500 µl of blood on 50 µl of ACD Add 600 µl of PBS, centrifuge once at 10ѓC, 1200 rpm ( Joan, GR 412) In Glass Centrifuge Tube add 100
with RNAse and 1 phenol or chloroform extraction followed by 1 ethanol precipitation with 2x 70% EtOH washes. Alternatively, use a Qiagen mini-prep kit. Resuspend miniprep in 50 µL of T.E. Use 3 µL per diagnostic digest. Pour 2 medium test gels
Native Chromatin Preparation and Illumina/Solexa Library Construction
(1X PBS containing 0.5 % bovine serum albumin, filtered) T4 DNA ligase (400 U/µL) and 10X buffer Taq polymerase (New England Biolabs) TaqMan PCR master mix (Applied Biosystems) (see Step 38) TE (1X, pH 7.4) Triton X-100
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