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文献和实验Two-hybrid analysis of genetic
mate to form diploids,the YPD plate is pressed to a new replica velvet and the impression is lifted with a plate containing diploid selection medium and an indicator like X-Gal.Blue patches (dark spots)on the X-Gal plate indicate that the lacZ reporter
Performing a hunt by interaction mating
of cfu can be sufficiently estimated from optical density (1 OD600 =~2 X 107 cells/ml). Under these conditions, ~10% of the cfu in the pretransformed library strain will mate with the bait strain. Thus, a complete screen of 107 library transformants
HeTube Mating with MSCV recipient
colonies and wet plates will make colonies run together. To calculate efficiency of the mating, count the colonies on the plate. # colonies X dilution X 10 = # colonies/ml a high efficiency is 107 colonies/ml
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