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文献和实验Transmigration of Human CD34+ Cells
to understand the mechanisms responsible for their homing and/or mobilization. In this chapter, we will describe the methodology utilized in our laboratory to evaluate migration of human CD34+ cells that contain HSC.
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
Generation of TransgenicT Cells from Human CD34+Cord Blood Cells
Hematopoietic stem cells (HSCs), progenitor cells capable of generating all peripheral blood cell lineages, are believed not to express lineage markers (Lin) found on the latter, nor CD38, but may or may not express CD34. Besides HSCs
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