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上海玉博生物科技有限公司
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文献和实验Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example
in incomplete Freund’s adjuvant, the respective antisera were collected and assayed by enzyme-linked immunosorbent assay (ELISA) and purified by affinity chromatography on peptides conjugated to SulfoLink Coupling Gel (Pierce). Mouse brain proteins were prepared
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
‐affinity peptide ligands of the WW domain of human and mouse YAP. Biol. Chem. 378:531‐537. Paige, L.A., Christensen, D.J., Grøn, H., Norris, J.D., Gottlin, E.B., Padilla, K.M
, immunoprecipitation is performed using anti‐FLAG M2 agarose (FLAG‐IP). The bound material is eluted with FLAG peptide and further purified by streptavidin affinity capture. The purified protein complexes are fractionated on SDS‐PAGE, and subjected to LC
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