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文献和实验Saturation Analysis of Ligand Binding Using a Centrifugation Procedure
assays is often unsuitable for radioligands with rapid dissociation rates. Rapid dissociation may result in the loss of significant amounts of bound ligand during the separation of bound from free ligand associated with filtration. The primary advantage
In Vitro Motility System to Study the Role of Motor Proteins in Receptor-Ligand Sorting
This chapter presents fluorescence microscope assays that can be used to study microtubule (MT)-based movement and receptor-ligand sorting in vitro. The strategy is to isolate endosomes in a concentrated active form and store them in frozen
about receptor function, particularly regarding the role of accessory proteins and/or membrane lipids in ligand binding.
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