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文献和实验Flexible Ligand Docking with Glide
Abstract Table of Contents Figures Literature Cited Abstract Glide is a ligand docking program
Isolation of murine splenocytes
attached (Protocol A only) frosted-end glass slides (x 2) (Protocol B only) 50ml conical tube (Protocol B only) Reagents DMEM-10 (about 20mL) 1L DMEM (with 4.5g/L glucose, L-glutamine, sodium pyruvate
Ligand Characterization Using Microphysiometry
of cells to the agonist and the pA2 value for the antagonist. Support protocols describe optimization of two aspects of this procedure: the duration of ligand exposure at a given concentration and the length of recovery time between the administration
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