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文献和实验Procedures for In Vitro Production of Bovine Embryos
maturation medium (111050-059)OncoImmune301-987-7881PhiPhiLux G1D2 (A304R1G-3)Pel Freez Biologicals 1-800-643-3426 pfbio@iso.netBovine steer serum (37999-0) Pierce Chemical 1-800-874-3723 CS@piercenet.comPCC-Pfree Concentrate (72290)Research Products
or gamma globulin are recommended)in 100 µl volume.See how to set up an assay for suggestions as to setting up the standards.5.Add 5 ml dye reagent and incubate 5 min.6.Measure the absorbance at 595 nm.AnalysisPrepare a standard curve of absorbance
. The Bradford assay is rather sensitive to bovine serum albumin, more so than "average" proteins, by about a factor of two. Immunoglogin G (IgG - gamma globulin) is the preferred protein standard. The addition of 1 M NaOH was suggested by Stoscheck (1990
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