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上海玉博生物科技有限公司
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文献和实验the correct amount of ethanol. 5. Apply the entire sample, including any precipitates that may form to a HiBind® RNA Mini column assembled in a clean 2 ml collecting tube (supplied). Close the cap gently. Centrifuge at 10,000 x g for 30 sec at room
, the volume of supernatant may vary. After transferring to a fresh tube, measure the volume and add the correct amount of ethanol. 5. Apply the entire mixture, including any precipitates that may form to a HiBind® RNA Midi column assembled in a 15.0 ml collection tube
Synthesis and Probing of Membrane-bound Peptide Arrays
Membrane blocking solution (MBS) Mix 20 ml of casein-based blocking buffer concentrate (No. SU-07-250; Sigma-Genosys), 80 ml of T-TBS (pH 8.0), and 5 g of sucrose; the resulting pH will be 7.0; store at 4°C.
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