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文献和实验such as EDTA, ammonia sulfate or Triton X-100 in particular are incompatible with the test. Bicinchoninine acid assay 562nm (BCA) This test represents a highly regarded alternative to the Lowry assay. It is easier to carry out and sensitivity
Quick and Easy Isolation of Genomic DNA from Yeast
Triton X-100 � 1% SDS, � 100 mM NaCl � 10 mM Tris-HCl, pH 8.0, � 1 mM EDTA
0.1ml ethidium bromide (10mg/ml). [It may be useful at this point to spin down any precipitate that has formed. This can interfere with the banding process later.] Transfer to ultracentrifuge tube (1/2 X 2 inches (13 X 51 mm) quick seal
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