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文献和实验96-well RNA In Situ Hybridization Protocol
. Incubate at room temperature (RT) for 10 min.11. Spin 13K for 15 min, drain well, then resuspend damp pellet in 50μl50% formamide / 50% TE pH 7.5 / 0.1% Tween 20.12. Dilute probes to 25ug/ml where appropriate. This is a 50X stock for hybridization in 96
Preparation of Mycoplasma chromosomal DNA in low melting agarose
1. Spin 1.5ml fresh cells for 3 minutes. 2.Wash the pellet once with TNE buffer. 3. Suspend the pellet well in 20 ul TNE buffer. 4. Add 2 ul proteinase K ( stock 10 ug/ul in water, -20 C). 5. Add 20 ul low melting agarose (1.6% in TNE buffer
96-well RNA In Situ Hybridization Protocol
96-well RNA In Situ Hybridization Protocol The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle (Chromosoma 98 (1989), p81), but adapted to allow the screening of 96 RNA probes
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