CycLex特约代理商|CY-1252 CycLex® NM

Chromatin IP (CHIP assay)

and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time:      2 x 1 ml CHIP lysis buffer      2 x 1 ml CHIP

Fastfilter Plasmid Midi Kit Spin Protocol

130 ul 5M NaCl and 2.2 ml room temperature isopropanol. Vortex to mix and centrifuge at >15,000 x g for 30 min at 4°C. Carefully decant the supernatant. 3. Wash DNA pellet once with 1 ml ice-cold 70% ethanol and centrifuge at > 15,000 x g for 10

Extraction of DNA using DNAzol® Reagent

the cells again and resuspend them in one volume of cold (4°C) hypotonic solution [20 mM Tris HCl (pH 8.0), 10 mM EDTA]. Pellet the cells at 4,000 rpm for 10 min (4°C). Discard the supernatant and add 1 ml DNAzol® per 1-3 × 107 cells. Lyse the cells