CycLex特约代理商|CY-1251V2 CycLex®

Random Primer DNA labeling

of the Percentage of Label Incorporation The percentage of incorporation is determined by DE-81 filter-binding assay. 1.Dilute 1µl of the reaction mix 1:100 with 0.2M EDTA or water. Spot 5µl of it on each of the two Whatman® DE-81 filters (1.5 x 1.5cm). 2.Dry

张峰手稿：基因工程技术之CRISPR-Cas9

. The protocol includes steps arrangedin order of actual experiments: selection of targets and cloning oftargeting constructs (Subheading 3.1 ), delivery into mammaliancells (Subheading 3.2 ), assay for genomic cleavage (Subheading 3.3 ),implementation

Whole genome amplification protocol for ChIP-chip

both.) Materials & Reagents ChIP sample, total chromatin WGA1 Sigma GenomePlex® Whole Genome Amplification (WGA) Kit WGA3 Sigma GenomePlex® WGA Reamplification Kit (comment 6) Reviewer Comments Reviewed