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文献和实验Protein Engineering of Interferon Alphas
differences in these proteins may be responsible for a significant variety of biological actions. Understanding the structure and function of human IFN-α is very important. Recombinant techniques are important tools for the production and modification of IFN
Assessment of Natural Killer Cell Responses to Human Cytomegalovirus-Infected Macrophages
. Herein, we describe an autologous assay system to assess NK cell responses to HCMV-infected macrophages which is based on flow cytometry to detect CD107a expression and interferon gamma (IFNγ) secretion. Further we established a simple method to handle HCMV-infected macrophages
, and for isolating, cryopreserving, and testing peripheral blood mononuclear cells for interferon-γ (IFN-γ) responses to capsid are described in detail.
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